Interaction of gelatin with a fluorescein-labeled 42-kDa chymotryptic fragment of fibronectin.
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چکیده
منابع مشابه
Interaction of gelatin with a fluorescein-labeled 42-kDa chymotryptic fragment of fibronectin.
A 42-kDa gelatin binding fragment of human plasma fibronectin was labeled with fluorescein and its fluid-phase interaction with gelatin was investigated. At 25 degrees C in 0.1 M Tris, 0.15 M NaCl, pH 7.3, a dissociation constant, Kd = 0.6 microM, was obtained from the dependence of fluorescence polarization on gelatin concentration. An identical value was obtained for the unlabeled fragment by...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85119-0